Gel-Free Proteomics
Methods and Protocols
Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursing research in this critical field.
| ISBN: | 9781493958191 |
|---|---|
| Sprache: | Englisch |
| Seitenzahl: | 400 |
| Produktart: | Kartoniert / Broschiert |
| Herausgeber: | Gevaert, Kris Vandekerckhove, Joël |
| Verlag: | Humana Press |
| Veröffentlicht: | 23.08.2016 |
| Untertitel: | Methods and Protocols |
| Schlagworte: | LPI hexalene glycosylated proteins isobaric peptide termini labeling (IPTL) mass spectrometry organelle proteomics phosphoproteomics proteome research |
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