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The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful  Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,  Intrinsically Disordered Proteins: Methods and Protocols  aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com.Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
ISBN: 9781071605233
Sprache: Englisch
Seitenzahl: 951
Produktart: Gebunden
Herausgeber: Kragelund, Birthe B. Skriver, Karen
Verlag: Springer US
Veröffentlicht: 22.07.2020
Untertitel: Methods and Protocols
Schlagworte: E. coli expression NMR SAXS data mass spectrometry post-translational modification

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