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The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.
ISBN: 9781071608098
Sprache: Englisch
Seitenzahl: 479
Produktart: Gebunden
Herausgeber: Cobaleda, César Sánchez-García, Isidro
Verlag: Springer US
Veröffentlicht: 10.11.2020
Untertitel: Methods and Protocols
Schlagworte: Animal models Cancer stem cells Hematopoietic stem cells Human leukemias LSCs Tissue culture

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